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課程名稱 |
遺傳學實驗
Genetics Lab. |
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開課學期 |
112-2 |
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授課對象 |
生命科學院 生命科學系 |
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授課教師 |
丁照棣 |
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課號 |
LS3008 |
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課程識別碼 |
B01 31120 |
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班次 |
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學分 |
1.0 |
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全/半年 |
半年 |
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必/選修 |
必修 |
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上課時間 |
星期三6,7,8(13:20~16:20) |
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上課地點 |
生科1225 |
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備註 |
F群選擇必修。
總人數上限：25人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
Genetics laboratory includes several hands-on experiments to introduce methods and analytical tools in genetics. The experiments include cytogenetics, mutant analyses, segregation assay, linkage analyses, molecular genetics, and population genetics. We integrate genetic approaches as a mini-project for students to generated small deletions by CRISP-Ca9 transgenic tools in Drosophila. In addition to experimental and analytical skills, students must record their experimental results in detail and write a full report. |
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課程目標 |
The ultimate goal for this course is to enhance students ability in independent research and in applying genetic approaches to real world questions. |
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課程要求 |
Fully attendance is required and active participation is expected. Family vacations and social events are unacceptable reasons for missing a lab. If you must miss a class due to a serious illness or family emergency, please contact instructors by e-mail or phone directly. |
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預期每週課後學習時數 |
2-6 hours |
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Office Hours |
每週三 12:30~14:00 備註： Instructors or TAs will be available at LSB 405 from 12:00 to 14:00 every Wednesday. Meeting at different times is also possible if an appointment is scheduled in advance. |
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指定閱讀 |
1. Ashburner M and Roote J. 2000. Culture of Drosophila: The Laboratory Setup. in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA. (https://cshprotocols.cshlp.org/content/2007/3/pdb.ip34.full)
2. Hales KG, Korey CA, Larracuente AM, Roberts DM. 2015. Genetics on the Fly: A Primer on the Drosophila Model System, Genetics 201: 815–842.(https://doi.org/10.1534/genetics.115.183392)
3. Roote J, Prokop A. 2013. How to Design a Genetic Mating Scheme: A Basic Training Package for Drosophila Genetics, G3 Genes|Genomes|Genetics 3: 353–358. (https://doi.org/10.1534/g3.112.004820) |
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參考書目 |
1. DNA from the beginning (https://www.dnaftb.org/)
2. Genetics: From Genes to Genomes By M. Goldberg, J. Fischer, L. Hood, L. Hartwell, C. Aquadro, L. Silver and A. E. Reynolds (McGraw Hill)
3. New to Flies (https://wiki.flybase.org/wiki/FlyBase:New_to_Flies)
4. An Introduction to Balancers (https://bdsc.indiana.edu/stocks/balancers/balancer_intro.html) |
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
Mini-Project |
50% |
Lab notes, Homework, Project updates/ discussions, Presentation, Final Report
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2. |
Weekly Projects |
50% |
Quizes and Homework
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針對學生困難提供學生調整方式 |
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上課形式 |
以錄影輔助 |
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作業繳交方式 |
學生與授課老師協議改以其他形式呈現 |
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考試形式 |
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其他 |
由師生雙方議定 |
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週次 |
日期 |
單元主題 |
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第0週 |
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Instruction to students/ Expectation from the instructors (課前須知/ 課程設計的期待) |
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第1週 |
2/21 |
Lab notebook/ Working with Drosophila: sexual dimorphism/ visible markers, balancers (實驗紀錄/ 果蠅實驗基礎：雌雄雙性型、形態標記、平衡品系) |
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第2週 |
2/28 |
Holiday (假日) |
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第3週 |
3/06 |
Cytogenetics: Meiosis/ crossover (Zea mays)
(細胞遺傳：減數分裂, 染色體互換 -- 玉米) |
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第4週 |
3/13 |
Cytogenetics: Mitosis, karyotype (onion/ shallot)
(細胞遺傳：有絲分裂, 染色體核型-- 紅蔥頭) |
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第5週 |
3/20 |
Mini-Project: Knock-down versus knock-out approaches/ Degin gRNAs of the target gene
(微專題：基因緘默與基因剔除/ gRNA 設計, G0) |
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第6週 |
3/27 |
Cytogenetics: Polytene chromosome, cytogenetic map
(細胞遺傳：多絲染色體/ 染色體圖譜) |
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第7週 |
4/03 |
Fungi genetics: Recombination versus gene conversion (Sordaria)
(真菌四分子觀察：重組與置換) |
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第8週 |
4/10 |
Human population genetics I: Genomic DNA extraction/ PCR
(人類族群遺傳分析 I：DNA 萃取與 PCR增幅) |
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第9週 |
4/17 |
Human population genetics II: Restriction analyses, allele frequencies, Hardy-Weinberg equilibrium/ mini-project updates
(人類族群遺傳分析 II：基因型分析、基因頻率計算、哈溫平衡/ 微專題實驗進展討論) |
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第10週 |
4/24 |
Quantitative Genetics: Drosophila bristle number as a quantitative trait (數量性狀遺傳分析：果蠅剛毛數目的數量性狀遺傳分析) |
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第11週 |
5/01 |
Molecular characterization of mutations: sequence analyses
(突變品系分子分析: 序列分析) |
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第12週 |
5/08 |
Molecular characterization of mutations: Fly DNA extraction, PCR
(突變品系分子分析：果蠅 DNA 萃取、PCR) |
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第13週 |
5/15 |
Molecular characterization of mutations: Gel electrophoresis, cleaning-up for sequencing reaction/ Mini-project updates
(突變品系分子分析：電泳分析、定序樣本製備/ 微專題實驗進展討論) |
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第14週 |
5/22 |
Mini-project updates/ mutant stocks
(微專題實驗期末討論/繳交突變品系) |
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第15週 |
5/29 |
Mini-project presentation/ mutant stocks
(微專題實驗期末報告/繳交突變品系) |
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第16週 |
6/05 |
[Optional] Mini-project Extension
(微專題實驗延伸、自由參加另約時間) |
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